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 | | From: | Gordon Couger | | Subject: | Re: Improving the confocal microscope images | | Date: | Sat, 11 Dec 2004 00:47:04 -0600 |
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 | madhu.balasubramanian@gmail.com wrote:
> Hi all,
>
> I need to improve the images obtained from the confocal microscope for
> contrast, reduce noise and reduce the out of focus information in the
> image. I tried deconvolution; but I'm not yet satisfied with the
> results. I would like to have pitch black background with only the
> specimen in the forground (is that not everyone want out of any
> imaging device?); I've tried high pass filters to remove any layers of
> light and few other filters for processing. But I would appreciate if
> anyone has other ideas/know other methods; also, please write if there
> could be anything that could be tried at the confocal itself.
>
> Thanks very much,
> Madhu.
I have successfully subtracted the background from an image of
an empty field form a image using ImageJ. I took an image of the
field with a blank slide and used the same exposure as i did for
them image.
I don't remember exactly what I did but using the
process>math>subtract to remove the blank image from the
complete one. It left a very thin image that had lost all the
uneven lighting dust and most of the artifacts from a 995
CoolPix camera.
I never was able to find a way to satisfactory add it to a
background to give an image with any dynamic range. But I am
sure that it could be done.
Good Luck
Gordon
Gordon Couger
I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org
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